Marker:Article Title: Immunopathogenesis of classical swine fever: role of monocytic cells
Article Snippet: .. Immunophenotyping of leucocytes This employed the following mAbs: anti-SWC1 [HB141, 76-6-7; American Type Culture Collection (ATCC), Rockville, MD], found on porcine monocytes; 18 anti-SWC3 (DH59; VMRD, Pullman, WA) porcine pan-myeloid cell marker; 18 anti-SWC9 (PM18-7, Dr Y. Kim, Finch University of Health Sciences, Chicago, IL), found on porcine Mφ but not monocytes; 17 and mAb My4 (Coulter-Clone, Instrumenten-Gesellschaft AG, Schlieren, Switzerland) against human and porcine CD14. .. 19 Incubations were performed for 20 min at 4° with the mAbs and for 15 min at 4° with phycoerythrin (PE)-conjugated antimouse immunoglobulin (DAKO, Glostrup, Denmark).
Article Title: Lipopolysaccharide and phorbol 12-myristate 13-acetate both impair monocyte differentiation, relating cellular function to virus susceptibility
Article Snippet: .. 4 , 5 , 10 Determination of differentiation antigen expression (surface and intracellular) These analyses employed the FACScan® Flow Cytometer (Becton Dickinson, Mountain View, CA) and the following monoclonal antibodies (mAb) for surface antigen expression: anti-SWC1 (76-6-7; American Type Culture Collection, Rockville, MD) found on porcine monocytes and T lymphocytes; 20 anti-SWC3 [DH59; Veterinary Medical Research and Development (VMRD), Pullman, WA] porcine pan-myeloid cell marker; 21 , 22 anti-SWC9 (PM18-7; kindly provided by Dr Y. Kim, Finch University of Health Sciences, Chicago, IL) found on porcine Mφ but not monocytes. .. 4 , 10 , 22 , 23 The tumour suppressor gene product p53 and the myeloperoxidase protein were the differentiation-susceptible intracellular antigens studied, 10 detected with fluorescein isothiocyanate (FITC)-conjugated anti-human p53 protein mAb DO-7, 24 and FITC-conjugated anti-human myeloperoxidase mAb MPO-7 25 (Dako, Copenhagen, Denmark).
Article Title: Lipopolysaccharide and phorbol 12-myristate 13-acetate both impair monocyte differentiation, relating cellular function to virus susceptibility
Article Snippet: .. These analyses employed the FACScan® Flow Cytometer (Becton Dickinson, Mountain View, CA) and the following monoclonal antibodies (mAb) for surface antigen expression: anti-SWC1 (76-6-7; American Type Culture Collection, Rockville, MD) found on porcine monocytes and T lymphocytes; 20 anti-SWC3 [DH59; Veterinary Medical Research and Development (VMRD), Pullman, WA] porcine pan-myeloid cell marker; 21 , 22 anti-SWC9 (PM18-7; kindly provided by Dr Y. Kim, Finch University of Health Sciences, Chicago, IL) found on porcine Mφ but not monocytes. .. 4 , 10 , 22 , 23 The tumour suppressor gene product p53 and the myeloperoxidase protein were the differentiation-susceptible intracellular antigens studied, 10 detected with fluorescein isothiocyanate (FITC)-conjugated anti-human p53 protein mAb DO-7, 24 and FITC-conjugated anti-human myeloperoxidase mAb MPO-7 25 (Dako, Copenhagen, Denmark).
Article Title: Immunopathogenesis of classical swine fever: role of monocytic cells
Article Snippet: .. This employed the following mAbs: anti-SWC1 [HB141, 76-6-7; American Type Culture Collection (ATCC), Rockville, MD], found on porcine monocytes; 18 anti-SWC3 (DH59; VMRD, Pullman, WA) porcine pan-myeloid cell marker; 18 anti-SWC9 (PM18-7, Dr Y. Kim, Finch University of Health Sciences, Chicago, IL), found on porcine Mφ but not monocytes; 17 and mAb My4 (Coulter-Clone, Instrumenten-Gesellschaft AG, Schlieren, Switzerland) against human and porcine CD14. .. 19 Incubations were performed for 20 min at 4° with the mAbs and for 15 min at 4° with phycoerythrin (PE)-conjugated antimouse immunoglobulin (DAKO, Glostrup, Denmark).
Expressing:Article Title: Lipopolysaccharide and phorbol 12-myristate 13-acetate both impair monocyte differentiation, relating cellular function to virus susceptibility
Article Snippet: .. 4 , 5 , 10 Determination of differentiation antigen expression (surface and intracellular) These analyses employed the FACScan® Flow Cytometer (Becton Dickinson, Mountain View, CA) and the following monoclonal antibodies (mAb) for surface antigen expression: anti-SWC1 (76-6-7; American Type Culture Collection, Rockville, MD) found on porcine monocytes and T lymphocytes; 20 anti-SWC3 [DH59; Veterinary Medical Research and Development (VMRD), Pullman, WA] porcine pan-myeloid cell marker; 21 , 22 anti-SWC9 (PM18-7; kindly provided by Dr Y. Kim, Finch University of Health Sciences, Chicago, IL) found on porcine Mφ but not monocytes. .. 4 , 10 , 22 , 23 The tumour suppressor gene product p53 and the myeloperoxidase protein were the differentiation-susceptible intracellular antigens studied, 10 detected with fluorescein isothiocyanate (FITC)-conjugated anti-human p53 protein mAb DO-7, 24 and FITC-conjugated anti-human myeloperoxidase mAb MPO-7 25 (Dako, Copenhagen, Denmark).
Article Title: Lipopolysaccharide and phorbol 12-myristate 13-acetate both impair monocyte differentiation, relating cellular function to virus susceptibility
Article Snippet: .. These analyses employed the FACScan® Flow Cytometer (Becton Dickinson, Mountain View, CA) and the following monoclonal antibodies (mAb) for surface antigen expression: anti-SWC1 (76-6-7; American Type Culture Collection, Rockville, MD) found on porcine monocytes and T lymphocytes; 20 anti-SWC3 [DH59; Veterinary Medical Research and Development (VMRD), Pullman, WA] porcine pan-myeloid cell marker; 21 , 22 anti-SWC9 (PM18-7; kindly provided by Dr Y. Kim, Finch University of Health Sciences, Chicago, IL) found on porcine Mφ but not monocytes. .. 4 , 10 , 22 , 23 The tumour suppressor gene product p53 and the myeloperoxidase protein were the differentiation-susceptible intracellular antigens studied, 10 detected with fluorescein isothiocyanate (FITC)-conjugated anti-human p53 protein mAb DO-7, 24 and FITC-conjugated anti-human myeloperoxidase mAb MPO-7 25 (Dako, Copenhagen, Denmark).
Flow Cytometry:Article Title: Lipopolysaccharide and phorbol 12-myristate 13-acetate both impair monocyte differentiation, relating cellular function to virus susceptibility
Article Snippet: .. 4 , 5 , 10 Determination of differentiation antigen expression (surface and intracellular) These analyses employed the FACScan® Flow Cytometer (Becton Dickinson, Mountain View, CA) and the following monoclonal antibodies (mAb) for surface antigen expression: anti-SWC1 (76-6-7; American Type Culture Collection, Rockville, MD) found on porcine monocytes and T lymphocytes; 20 anti-SWC3 [DH59; Veterinary Medical Research and Development (VMRD), Pullman, WA] porcine pan-myeloid cell marker; 21 , 22 anti-SWC9 (PM18-7; kindly provided by Dr Y. Kim, Finch University of Health Sciences, Chicago, IL) found on porcine Mφ but not monocytes. .. 4 , 10 , 22 , 23 The tumour suppressor gene product p53 and the myeloperoxidase protein were the differentiation-susceptible intracellular antigens studied, 10 detected with fluorescein isothiocyanate (FITC)-conjugated anti-human p53 protein mAb DO-7, 24 and FITC-conjugated anti-human myeloperoxidase mAb MPO-7 25 (Dako, Copenhagen, Denmark).
Article Title: Lipopolysaccharide and phorbol 12-myristate 13-acetate both impair monocyte differentiation, relating cellular function to virus susceptibility
Article Snippet: .. These analyses employed the FACScan® Flow Cytometer (Becton Dickinson, Mountain View, CA) and the following monoclonal antibodies (mAb) for surface antigen expression: anti-SWC1 (76-6-7; American Type Culture Collection, Rockville, MD) found on porcine monocytes and T lymphocytes; 20 anti-SWC3 [DH59; Veterinary Medical Research and Development (VMRD), Pullman, WA] porcine pan-myeloid cell marker; 21 , 22 anti-SWC9 (PM18-7; kindly provided by Dr Y. Kim, Finch University of Health Sciences, Chicago, IL) found on porcine Mφ but not monocytes. .. 4 , 10 , 22 , 23 The tumour suppressor gene product p53 and the myeloperoxidase protein were the differentiation-susceptible intracellular antigens studied, 10 detected with fluorescein isothiocyanate (FITC)-conjugated anti-human p53 protein mAb DO-7, 24 and FITC-conjugated anti-human myeloperoxidase mAb MPO-7 25 (Dako, Copenhagen, Denmark).
Bioprocessing:Article Title: Lipopolysaccharide and phorbol 12-myristate 13-acetate both impair monocyte differentiation, relating cellular function to virus susceptibility
Article Snippet: .. 4 , 5 , 10 Determination of differentiation antigen expression (surface and intracellular) These analyses employed the FACScan® Flow Cytometer (Becton Dickinson, Mountain View, CA) and the following monoclonal antibodies (mAb) for surface antigen expression: anti-SWC1 (76-6-7; American Type Culture Collection, Rockville, MD) found on porcine monocytes and T lymphocytes; 20 anti-SWC3 [DH59; Veterinary Medical Research and Development (VMRD), Pullman, WA] porcine pan-myeloid cell marker; 21 , 22 anti-SWC9 (PM18-7; kindly provided by Dr Y. Kim, Finch University of Health Sciences, Chicago, IL) found on porcine Mφ but not monocytes. .. 4 , 10 , 22 , 23 The tumour suppressor gene product p53 and the myeloperoxidase protein were the differentiation-susceptible intracellular antigens studied, 10 detected with fluorescein isothiocyanate (FITC)-conjugated anti-human p53 protein mAb DO-7, 24 and FITC-conjugated anti-human myeloperoxidase mAb MPO-7 25 (Dako, Copenhagen, Denmark).
Article Title: Lipopolysaccharide and phorbol 12-myristate 13-acetate both impair monocyte differentiation, relating cellular function to virus susceptibility
Article Snippet: .. These analyses employed the FACScan® Flow Cytometer (Becton Dickinson, Mountain View, CA) and the following monoclonal antibodies (mAb) for surface antigen expression: anti-SWC1 (76-6-7; American Type Culture Collection, Rockville, MD) found on porcine monocytes and T lymphocytes; 20 anti-SWC3 [DH59; Veterinary Medical Research and Development (VMRD), Pullman, WA] porcine pan-myeloid cell marker; 21 , 22 anti-SWC9 (PM18-7; kindly provided by Dr Y. Kim, Finch University of Health Sciences, Chicago, IL) found on porcine Mφ but not monocytes. .. 4 , 10 , 22 , 23 The tumour suppressor gene product p53 and the myeloperoxidase protein were the differentiation-susceptible intracellular antigens studied, 10 detected with fluorescein isothiocyanate (FITC)-conjugated anti-human p53 protein mAb DO-7, 24 and FITC-conjugated anti-human myeloperoxidase mAb MPO-7 25 (Dako, Copenhagen, Denmark).
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